The Saccharomyces cerevisiae Actin Patch Protein App1p Is a Phosphatidate Phosphatase Enzyme

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The Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme.

BACKGROUND Phosphatidate phosphatase (PAP) plays diverse roles in lipid metabolism and cell signaling. RESULTS A novel yeast PAP is identified as the actin patch protein encoded by APP1. CONCLUSION APP1 and other known genes (PAH1, DPP1, LPP1) are responsible for all detectable PAP activity in yeast. SIGNIFICANCE Identification of App1p as a PAP enzyme will facilitate the understanding of...

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Characterization of the yeast actin patch protein App1p phosphatidate phosphatase.

Yeast App1p is a phosphatidate phosphatase (PAP) that associates with endocytic proteins at cortical actin patches. App1p, which catalyzes the conversion of phosphatidate (PA) to diacylglycerol, is unique among Mg(2+)-dependent PAP enzymes in that its reaction is not involved with de novo lipid synthesis. Instead, App1p PAP is thought to play a role in endocytosis because its substrate and prod...

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The Saccharomyces cerevisiae Arf3 protein is involved in actin cable and cortical patch formation.

We show that Arf3p, a member of the ADP ribosylation family, is involved in the organization of actin cables and cortical patches in Saccharomyces cerevisiae. Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects in these cells. Cells deficient for the cortical patch proteins Las17p and Vrp...

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Regulation of phosphatidate phosphatase activity from the yeast Saccharomyces cerevisiae by sphingoid bases.

The regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by sphingoid bases was examined using Triton X-100/lipid-mixed micelles. Sphingosine, phytosphingosine, and sphinganine inhibited purified preparations of the 104- and 45-kDa forms of phosphatidate phosphatase in a dose-dependent manner. The structu...

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The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton

Arp2p is an essential yeast actin-related protein. Disruption of the corresponding ARP2 gene leads to a terminal phenotype characterized by the presence of a single large bud. Thus, Arp2p may be important for a late stage of the cell cycle (Schwob, E., and R.P. Martin, 1992. Nature (Lond.). 355:179-182). We have localized Arp2p by indirect immunofluorescence. Specific peptide antibodies reveale...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2012

ISSN: 0021-9258

DOI: 10.1074/jbc.m112.421776